Preparative Density Gradient Centrifugations
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منابع مشابه
Rabbit skeletal muscle protein phosphatase(s). Identity of phosphorylase and synthase phosphatase and interconversion to the ATP-Mg-dependent enzyme form.
A high molecular weight protein phosphatase was purified from rabbit skeletal muscle displaying activity toward phosphorylase Q and synthase b isolated from the same tissue. The extensively purified enzyme was shown to have a M, = 260,000 by gel filtration and dissociated in sucrose density gradient centrifugations into a M, = 70,000 species. The 70,000-dalton enzyme form, resulting from prepar...
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There are numerous techniques for sampling density gradients in experiments involving the preparative ultracentrifuge. Among the sampling procedures used are: drawing liquid samples from the top of the density gradient with modified pipettes (J. B. Sinclair et al., Phytopathology 47:372, 1957), displacement through the top of the density gradient (E. Huff, H. Oxley, and C. S. Silverman, J. Bact...
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